Characterisitics and gene cloning of phospholipase D of the psychrophile, Shewanella sp |
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Authors: | Tsuruta Hiroki Hayashi Ryuhei Ohkawa Hiroyuki Ohkatsu Nobuhide Morimoto Masakazu Nishimoto Kaname Santou Sigeyuki Aizono Yasuo |
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Institution: | Headquarters for Innovative Cooperation and Development, Kobe University, Japan. tsuruta@kobe-u.ac.jp |
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Abstract: | Phospholipase D, with a molecular mass of 64 kDa, was purified from the psychrophile, Shewanella sp. The enzyme showed maximal activity at pH 7.8 and 40 degrees C in the presence of the Ca2+-ion, and its activity at 10 degrees C was 6.5% of maximum. The enzyme exhibited high activity to the non-micelle form of phosphatidylcholine in an aqueous solution containing water miscible alcohols such as methanol, ethanol, iso-propanol, and n-propanol. Nucleotide sequencing of the enzyme gene yielded a deduced amino acid sequence, which showed 36.2% identity to that of Streptomyces chromofuscus phospholipase D alone. The low sequence similarity to other phospholipase D enzymes suggests that the purified enzyme might be a novel phospholipase D. |
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