Detection of transcribable recombination products following conjugation in rec+, reCB- and recC-strains of Escherichia coli K12 |
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| Authors: | E A Birge K B Low |
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| Institution: | Radiobiology Laboratories, Yale University Medical School 333 Cedar Street, New Haven, Conn. 06510, U.S.A. |
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| Abstract: | By crossing Hfr and F? strains of Escherichia coli which carry non-identical (but non-complementing) lacZ? mutations, the detection of β-galactosidase produced from LacZ+ recombination products is possible, beginning 60 minutes after transfer of the Hfr lac? allele. This system was used to show that when the F? cells carry recB?, almost normal amounts of LacZ+ enzyme are formed even though the number of viable recombinants is less than 1% of the Rec+ level. A similar result is found when the F? cells carry recC?. In contrast, LacZ+ enzyme activity is not detected either when RecA? F? cells are used or in a stable RecA? merodiploid carrying the two lacZ? alleles. |
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