Aspartate aminotransferase isozymes in plants: Comparison of two staining methods in polyacrylamide gels |
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Authors: | J Stejskal |
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Institution: | 1. OSEVA, Research Institute of Technical Crops and Legumes, 787 01, Sumperk, Czech Republic
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Abstract: | Two staining methods for aspartate aminotransferase were compared after electrophoretic resolution of its isozymes in polyacrylamide gels. The first one uses L-aspartic acid and Fast Blue BB salt (classical method), the second uses L-cysteine sulfinic acid and a redox system with phenazine methosulfate and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide. The seeds of pea, horse bean and soybean were used as a model plant source of the enzyme. The staining method with L-cysteine sulfinic acid is very reliable and more sensitive than the Fast Blue BB method and allows detection at very low isozyme activities in the gel. |
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