Reporter enzymes for the study of promoter activity |
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Authors: | Karen Pardy |
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Affiliation: | 1. 9 Velindre Place, Whitchurch, Cardiff, CF4 2AN, Wales, UK
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Abstract: | This article describes the use of three reporter enzymes used to study promoter activity in transgenic animals. Chloramphenicol acetyl transferase may be assayed by a nonchromatographic method that is rapid and sensitive. β-Galactosidase is measured by a photometric assay and luciferase is assayed by measuring the emission of light using a luminometer. The relative merits of each enzyme is discussed. Ths use of reporter enzymes provides a rapid and sensitive method for analysis of transgene expression. |
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