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Detection of plum pox virus in leaves and aphids by SIBA and DAS-ELISA assays
Authors:J Kotúč  G Deborre
Institution:1. Institute of Experimental Phytopathology and Entomology, Slovak Academy of Science, 900 28, Ivanka pri Dunaji, Slovak Republic
2. Institute for Plant Protection in Fruit Crops, Biologische Bundesanstalt Schwabenheimer Str. 101, D-6915, Dossenheim, Germany
Abstract:The slot-immunobinding assay (SIBA) was adapted for detection of plum pox virus (PPV) and compared with DAS-ELISA. SIBA was easy to perform and as sensitive as DAS-ELISA in detection of various PPV isolates in herbaceous and woody plants, but not in aphids (Myzus persicae).
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