Detection of proteins and sialoglycoproteins in polyacrylamide gels using eosin Y stain |
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| Authors: | Fan Lin Gary E. Wise |
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| Affiliation: | 1. Department of Anatomy and Cell Biology, School of Veterinary Medicine, Louisiana State University, 70803, Baton Rouge, LA
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| Abstract: | An eosin Y staining technique that permits detection of various proteins, including membrane sialoglycoproteins, in polyacrylamide gels is described. The sensitivity of the eosin Y staining method is comparable to silver staining. In addition, there is an added advantage of the antigen icily of the stained proteins being retained in a Western blot. Details of the procedure to obtain optimal staining results are described. |
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