Studies on the distribution of 14C-malformin A in major fractions of Phaseolus vulgaris L.

The distribution pattern of ¹⁴C-malformin in major fractionsof Phaseolus vulgaris L. seedlings shifted during water treatmentin the absence of malformin. From these shifts, and by comparisonof the ¹⁴C distribution patterns at the base and top of theseedlings, it was concluded that some ¹⁴C-malformin enters thecell and proceeds to the cell wall via intermediate compounds.As a working hypothesis it was suggested that in roots ¹⁴C-malforminfirst appears in a soluble "small molecules" fraction, bindsto a soluble protein fraction, and proceeds via die wall lipidfraction to the wall itself. Direct binding of some ¹⁴C-malforminto the wall fraction was not precluded. In leaves, the pathwayof ¹⁴C-malformin to the cell wall was similar in some respectsto that in roots. ¹ Present address: American Cyanamid, P.O. Box 400, Princeton,New Jersey 08540, U.S.A. (Received January 21, 1976; )