Restriction Endonuclease (REA) Typing of Clostridium difficile Isolates from South America |
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| Affiliation: | 1. Department of Preventive Medicine, University of Mississippi Medical Center, Jackson, Mississippi;2. Department of Pediatrics, University of Mississippi Medical Center, Jackson, Mississippi;3. Office of the Provost, University of Houston, Houston, Texas;4. College of Medicine, University of Houston, Houston, Texas;5. Department of Data Science, University of Mississippi Medical Center, Jackson, Mississippi;6. Program for Maternal Health Equity, Center for Urban Bioethics, Lewis Katz School of Medicine at Temple University, Philadelphia, Pennsylvania;7. Department of Population Health Science, University of Mississippi Medical Center, Jackson, Mississippi;8. Department of Implementation Science, Division of Public Health Sciences, Wake Forest School of Medicine, Winston-Salem, North Carolina;1. Tropical Metabolism Research Unit, Caribbean Institute for Health Research (formerly Tropical Medicine Research Institute), The University of the West Indies, Mona, Jamaica;2. Department of Basic Medical Sciences, The University of the West Indies, Mona, Jamaica |
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| Abstract: | Little is known about the genetic relationship of pathogenic strains of Clostridium difficile from various parts of the world. We used Hind III restriction digestion of whole DNA to type isolates of C. difficile form hospitals in Argentina and Chile. The restriction pattern type of these South American isolates was classified according to the existing REA library of over 400 distinct REA types in over 90 groups, based on typing of more than 6000 clinical isolates. A total of 22 isolates of C. difficile was obtained and typed. The majority of isolates were matched to groups previously seen in North American and Europe. Three toxigenic groups, R, F, and Y, predominated in the isolates. Five isolates were found to be non-toxigenic and three belonged to group M, the most common non-toxigenic group found in North America. Several identical types were recovered from different hospitals, and types of the same group were found in both Buenos Aires and Santiago. |
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