Enterotoxin-producing Bacteroides fragilis (ETBF) Strains in Stool Samples Submitted for Testing ofClostridium difficile and its Toxins |
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| Affiliation: | 1. Department of Medical Microbiology, Institute of Biostructure, Warsaw Medical School, Chalubinski street 5, 02-004, Warsaw, Poland;2. Department of Medical Microbiology and Infectious Diseases, University Hospital, Rotterdam, The Netherlands;1. Department of Immunology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran;2. Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran;1. Department of Clinical Science, University of Bergen, N-5021 Bergen, Norway;2. Department of Microbiology and Immunology, Muhimbili University of Health and Allied Sciences, Tanzania;3. Centre for Tropical Infectious Diseases, Department of Medicine, Haukeland University Hospital, Bergen, Norway;4. Department of Microbiology, Haukeland University Hospital, Bergen, Norway;5. Division of Infectious Disease Control, Norwegian Institute of Public Health, Oslo, Norway;6. Department of Medicine, Haukeland University Hospital, Bergen, Norway;1. Zhujiang Hospital of Southern Medical University, Guangzhou 510282, Guangdong, PR China;2. Department of Orthopaedics, Zhujiang Hospital, Southern Medical University, Guanzhou 510280, Guangdong, PR China |
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| Abstract: | Fifty faecal samples of patients suspected of having diarrhoea associated with Clostridium difficile were studied. Toxins of C. difficile were tested in vivo directly from the faecal sample using Toxin Detection Kits (Oxoid) to detect toxin A and primers for detection genes of Toxin A and B in a PCR test. The same samples were tested for B. fragilis enterotoxin gene directly from the faecal sample using special primers and a PCR test. Samples were inoculated onto selective media for C. difficile (CCCA) and B. fragilis (BBE) for isolation of bacteria.In vitro Toxin A of C. difficile in culture was tested using a C. difficile toxin A immunoassay (Oxoid, U.K. test and Toxin B of C. difficile was tested by using the McCoy cell line. C. difficile toxin A and B genes were determined in DNA of isolated strains using special primers and a PCR reaction. The enterotoxin production in B. fragilis strains was tested on the human carcinoma cell line HT29/C1. The presence of fragilysin gene was detected using a special pair of primers and a PCR reaction. Toxinogenic strains of C. difficile and enterotoxigenic Bacteroides fragilis (ETBF) strains were isolated from the same samples. |
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