Calcium regulation of nucleocytoplasmic transport |
| |
Authors: | Ashapurna Sarma Weidong Yang |
| |
Affiliation: | Department of Biological Sciences, Center for Photochemical Sciences, Bowling Green State University, Bowling Green, OH 43403, USA |
| |
Abstract: | Bidirectional trafficking of macromolecules between the cytoplasm and the nucleus is mediated by the nuclear pore complexes (NPCs) embedded in the nuclear envelope (NE) of eukaryotic cell. The NPC functions as the sole pathway to allow for the passive diffusion of small molecules and the facilitated translocation of larger molecules. Evidence shows that these two transport modes and the conformation of NPC can be regulated by calcium stored in the lumen of nuclear envelope and endoplasmic reticulum. However, the mechanism of calcium regulation remains poorly understood. In this review, we integrate data on the observations of calcium-regulated structure and function of the NPC over the past years. Furthermore, we highlight challenges in the measurements of dynamic conformational changes and transient transport kinetics in the NPC. Finally, an innovative imaging approach, single-molecule super-resolution fluorescence microscopy, is introduced and expected to provide more insights into the mechanism of calcium-regulated nucleocytoplasmic transport. |
| |
Keywords: | nuclear envelope nuclear pore complex nucleocytoplasmic transport calcium stores single-molecule fluorescence microscopy |
|
| 点击此处可从《蛋白质与细胞》浏览原始摘要信息 |
|
点击此处可从《蛋白质与细胞》下载全文 |
|