Hrs inhibits citron kinase-mediated HIV-1 budding via its FYVE domain |
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Authors: | Jiwei Ding Lishan Su Guangxia Gao |
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Affiliation: | 1. Key Laboratory of Infection and Immunity, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China; 2. Graduate University of Chinese Academy of Sciences, Beijing 100039, China; 3. The University of North Carolina, Chapel Hill, NC 27599-7295, USA |
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Abstract: | Hepatocyte growth factor-regulated tyrosine kinase substrate (Hrs) is a key component of the endosomal sorting complexes required for transport and has been demonstrated to play a regulatory role in endocytosis/exocytosis and the accumulation of internal vesicles in multivesicular bodies. Citron kinase is a Ser/The kinase that we previously reported to enhance human immunodeficiency virus type 1 (HIV-1) virion production. However, the relationship between Hrs and citron kinase in HIV-1 production remains elusive. Here, we report that Hrs interacts with citron kinase via its FYVE domain. Overexpression of Hrs or the FYVE domain resulted in a significant decrease in HIV-1 virion production. Depletion of Hrs by RNA interference in HEK293T cells increased HIV-1 virion production and enhanced the activity of citron kinase. These data suggest that Hrs inhibits HIV-1 production by inhibiting citron kinase-mediated exocytosis. |
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Keywords: | citron kinase Hrs HIV-1 budding |
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