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The role of Pvs28 in sporozoite development in Anopheles sinensis and its longevity in BALB/c mice |
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| Authors: | Tong-Soo Kim Hyung-Hwan Kim Sung-Ung Moon Sun-Sim Lee E-Hyun Shin Chang-Mi Oh Yoon-Joong Kang Do-Kyung Kim Youngjoo Sohn Hyuck Kim Hyeong-Woo Lee |
| Institution: | aDepartment of Parasitology, College of Medicine, Inha University, Incheon 405-751, Republic of Korea;bVascular Medicine Research Unit, Brigham and Women’s Hospital, Harvard Medical School, Cambridge, MA 02139, USA;cDivision of Malaria and Parasitic Diseases, National Institute of Health, Center for Disease Control and Prevention, Seoul 122-701, Republic of Korea;dDivision of Medical Entomology, National Institute of Health, Centers for Disease Control and Prevention, Seoul 122-701, Republic of Korea;eFaculty of Biomedical Engineering, Jungwon University, Goesan 367-805, Republic of Korea;fInternational Research Center of Bioscience and Biotechnology, Goesan 367-805, Republic of Korea;gDepartment of Gynecology, College of Oriental Medicine, Sangji University, Wonju 220-717, Republic of Korea;hDepartment of Pathology, University of Florida, J-566, 1600 SW Archer Road, Gainesville, FL 32610, USA |
| Abstract: | To develop a vivax malaria vaccine for blocking malarial transmission, the ookinete surface protein Pvs28 was cloned from Korean malaria patients using polymerase chain reaction. The Pvs28 gene consists of 726 bp and encodes 241 amino acids. It was subcloned into the expression vector pQE30 and expressed in Escherichia coli. The expressed recombinant protein, rPvs28, has a molecular weight of about 28 kDa in SDS–PAGE analysis. A monoclonal antibody against rPvs28 was produced using BALB/c mice. It inhibited sporozoite development in Anopheles sinensis mosquitoes (n = 81) which is one of the malaria vectors in Korea, with relatively high antibody titer against rPv28 persisting for more than 6 months. These results indicate that rPvs28 induces an immune response in mice that effectively blocks sporozoite development in mosquitoes. Therefore it could be a vaccine candidate for preventing vivax malaria in Korea. |
| Keywords: | Plasmodium vivax Ookinete surface protein Transmission-blocking vaccine Monoclonal antibody Longevity |
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