Trypanosoma cruzi: Multiple actin isovariants are observed along different developmental stages |
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Authors: | Ana Marí a Cevallos,Yayoi X. Segura-Kato,Horacio Merchant-Larios,Rebeca Manning-Cela,Luis Alberto Herná ndez-Osorio,Claudia Má rquez-Dueñ as,Javier R. Ambrosio,Olivia Reynoso-Ducoing,Roberto Herná ndez |
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Affiliation: | aDepartamento de Biología Molecular y Biotecnología, Universidad Nacional Autónoma de México, Apartado Postal 70-228, México, DF, Mexico;bDepartamento de Biología Celular y Fisiología del Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Apartado Postal 70-228, México, DF, Mexico;cDepartamento de Microbiología y Parasitología de la Facultad de Medicina, Universidad Nacional Autónoma de México, Apartado Postal 4510, México, DF, Mexico;dDepartamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados del IPN, Apartado Postal 14–740, 07000 México, DF, Mexico;eFacultad de Medicina y Cirugía, Universidad Autónoma Benito Juárez de Oaxaca, Oaxaca, Mexico |
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Abstract: | The expression and biological role of actin during the Trypanosoma cruzi life cycle remains largely unknown. Polyclonal antibodies against a recombinant T. cruzi actin protein were used to confirm its expression in epimastigotes, trypomastigotes, and amastigotes. Although the overall levels of expression were similar, clear differences in the subcellular distribution of actin among the developmental stages were identified. The existence of five actin variants in each developmental stage with distinct patterns of expression were uncovered by immunoblotting of protein extracts separated 2D-SDS gels. The isoelectric points of the actin variants in epimastigotes ranged from 4.45 to 4.9, whereas they ranged from 4.9 to 5.24 in trypomastigotes and amastigotes. To determine if the actin variants found could represent previously unidentified actins, we performed a genomic survey of the T. cruzi GeneDB database and found 12 independent loci encoding for a diverse group of actins and actin-like proteins that are conserved among trypanosomatids. |
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Keywords: | Trypomastigote Epimastigote Amastigote Kinetoplastid Trypanosomatid Actin isoforms Actin-like protein Actin-related protein |
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