Numb modulates intestinal epithelial cells toward goblet cell phenotype by inhibiting the Notch signaling pathway |
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Authors: | Yang Yongtao Zhu Rong Bai Jianying Zhang Xin Tian Yin Li Xiaohuan Peng Zhihong He Yonghong Chen Lei Ji Qing Chen Wensheng Fang Dianchun Wang Rongquan |
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Affiliation: | aDepartment of Gastroenterology, Southwest Hospital, Third Military Medical University, Chongqing 400038, P. R. of China |
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Abstract: | Numb was originally identified as an important cell fate determinant that is asymmetrically inherited during mitosis and controls the fate of sibling cells by inhibiting the Notch signaling pathway in neural tissue. The small intestinal epithelium originates from the division of stem cells that reside in the crypt, which further differentiate into goblet cells, absorptive cells, paneth cells, and enteroendocrine cells. However, Numb's involvement in the differentiation process of intestinal epithelium is largely unknown. In the present study, we confirm that both the Numb mRNA and protein isoforms are expressed in adult mouse intestinal mucosa. Numb protein is ubiquitously expressed throughout the crypt–villus axis of the small intestinal epithelium and is mainly localized to the cytoplasmic membrane. Down-regulation of endogenous Numb using RNA interference in cultured intestinal LS174T cells increased Notch signaling, leading to the up-regulation of Hes1 and the down-regulation of Hath1. Knockdown of Numb alleviated MUC2 protein expression and led to loss of the goblet cell phenotype in LS174Tl cells. Our results provide the first evidence that Numb, an important cell fate determinant, modulates intestinal epithelial cells towards the goblet cell phenotype by inhibiting the Notch signaling pathway. |
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Keywords: | Abbreviations: NICD, Notch intracellular domain PBS, phosphate buffered saline PTB, amino-terminal phosphotyrosine-binding domain PRR, carboxyl-terminal praline rich region |
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