| 莲藕染色体上荧光原位杂交方法的初探 |
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| 引用本文： | 刁英. 莲藕染色体上荧光原位杂交方法的初探[J]. 氨基酸和生物资源, 2004, 26(2): 23-25. DOI: 10.3969/j.issn.1006-8376.2004.02.009 |
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| 作者姓名： | 刁英 |
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| 作者单位： | 武汉大学发育生物学教育部重点实验室;渝西学院生命科学系,重庆永川,402168;武汉大学莲藕中心,武汉,430072 |
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| 基金项目： | 国家科技攻关项目，湖北省科技攻关项目，湖北省武汉市科技攻关项目 |
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| 摘 要： | 用酶解滴片法制备莲的染色体标本片,在传统的荧光原位杂交(FISH)的方法上进行改进,得到了一种适合于莲的高效荧光原位杂交方法,为莲的分子细胞遗传学研究提供技术上的帮助。
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| 关 键 词： | 莲 酶解滴片法 染色体 FISH |
| 文章编号： | 1006-8376(2004)02-0023-03 |
| 修稿时间： | 2004-02-10 |
A Study of the FISH Protocol on Lotus Chromosomes |
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| DIAO Ying. A Study of the FISH Protocol on Lotus Chromosomes[J]. Amino Acids & Biotic Resources, 2004, 26(2): 23-25. DOI: 10.3969/j.issn.1006-8376.2004.02.009 |
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| Authors: | DIAO Ying |
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| Affiliation: | ,DIAO Ying( Key Laboratory of MOE for Developmental Biology, Wuhan University,Biology Department, West Chongqing University,Yongchuan Chongqing 402168Lotus Research Center, Wuhan University, Wuhan 430072, China) |
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| Abstract: | The chromosomes of lotus were prepared by drop - spreading technique. And a efficient FISH method based on the original method was determined. This method could improve the study of lotus molecular cytogenet-ics. |
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| Keywords: | FISH |
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