Genetic mapping in pea. 1. RAPD-based genetic linkage map of Pisum sativum |
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| Authors: | V Laucou K Haurogné N Ellis C Rameau |
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| Institution: | (1) Station de Génétique et d’Amélioration des Plantes, Institut National de la Recherche Agronomique, Route de Saint-Cyr, 78026 Versailles Cedex, France Fax :+3-1-30 83 33 19 E-mail: rameau@versailles.inra.fr, FR;(2) Department of Applied Genetics, John Innes Centre, Colney Lane, Norwich NR4 7UH, UK, GB |
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| Abstract: | A genetic linkage map of Pisum sativum L. was constructed based primarily on RAPD markers that were carefully selected for their reproducibility and scored in a
population of 139 recombinant inbred lines (RILs). The mapping population was derived from a cross between a protein-rich
dry-seed cultivar ‘Térèse’ and an increased branching mutant (K586) obtained from the pea cultivar ‘Torsdag’. The map currently
comprises nine linkage groups with two groups comprising only 6 markers (n=7 in pea) and covers 1139 cM. This RAPD-based map has been aligned with the map based on the (JI281×JI399) RILs population
that currently includes 355 markers in seven linkage groups covering 1881 cM. The difference in map lengths is discussed.
For this alignment 7 RFLPs, 23 RAPD markers, the morphological marker le and the PCR marker corresponding to the gene Uni were used as common markers and scored in both populations.
Received: 13 March 1998 / Accepted: 29 April 1998 |
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| Keywords: | Linkage mapping Pisum sativum Random amplified polymorphic DNA Recombinant inbred lines |
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