Effects of hepatocyte growth factor and platelet-derived growth factor on the repair of meniscal defects in vitro |
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Authors: | Madhu M Bhargava Chisa Hidaka Jo A Hannafin Stephen Doty Russell F Warren |
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Institution: | (1) Laboratory for Soft Tissue Research, Hospital for Special Surgery, 535 East 70th Street, 10021 New York, New York |
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Abstract: | Summary Injuries to the avascular region of the meniscus occur frequently and may be difficult to repair. This study was designed
to determine whether growth factors could diffuse from a collagen sponge or a collagen gel into meniscal tissue and stimulate
healing of defects using an in vitro model. The diffusion of platelet-derived growth factor (PDGF) from the collagen carriers
into the medium was rapid with approximately 50% being released from the collagen sponge within the first hour. After 5 d
of incubation, 8% of the PDGF was present in the meniscus, 11% in the collagen sponge, and 62% had been released into the
medium. Similar results were obtained when a collagen gel was used as a carrier. Histological evaluation of the meniscal explants
after 2 wk in culture revealed extensive proteoglycan staining in the areas surrounding defects treated with either hepatocyte
growth factor (HGF) or PDGF compared with controls without growth factor. The HGF-PDGF treatment resulted in alignment and
migration of meniscal cells toward the defect, which was not observed in untreated controls. At 3–7 d, increased number of
cells were observed in defects treated with collagen gels (but not the sponge) with PDGF-HGF. At 4 wk, combined HGF-PDGF treatment
resulted in the formation of tissue with birefringence by polarized microscopy, suggestive of organized collagen. The data
suggest that use of specific PDGF-HGF may enhance the repair of meniscal injuries. |
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Keywords: | cytokines meniscus injury healing |
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