Multiplexed measurement of serum antibodies using an array biosensor |
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Authors: | Moreno-Bondi Maria C Taitt Chris Rowe Shriver-Lake Lisa C Ligler Frances S |
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Institution: | Department Química Analítica, Facultad de Química, Universidad Complutense, 28040 Madrid, Spain. |
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Abstract: | The array biosensor provides the capability for simultaneously measuring titers of antibody against multiple antigens. Human antibodies against four different targets, tetanus toxin, diphtheria toxin, staphylococcal enterotoxin B (SEB) and hepatitis B, were measured simultaneously in sera from eight different donors in a single assay and titers were determined. The assays could measure amounts of bound antibody as low as approximately 100 fg. Each individual serum exhibited a different pattern of reactivity against the four target antigens. Applications of this biosensor capability include monitoring for exposure to pathogens and for efficacy of vaccination. |
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