| 酵母N-糖基化工程研究进展 |
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| 引用本文： | 詹洁,吴军.酵母N-糖基化工程研究进展[J].生物技术通讯,2004,15(3):272-274. |
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| 作者姓名： | 詹洁 吴军 |
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| 作者单位： | 军事医学科学院,生物工程研究所,北京,100071 |
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| 摘 要： | 酵母表达系统可用来生产具生物活性的重组糖蛋白，但其在N-糖基化过程中会生成高甘露糖型糖链。通过引入相关的甘露糖苷酶和糖基转移酶基因、切断酵母自身的高甘露糖链形成通道能够改变酵母宿主N-糖基化的类型。本对酵母N-糖基化工程的研究状况、最新进展及存在问题作简要阐述。
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| 关 键 词： | 酵母 N-糖基化 高甘露糖型 甘露糖苷酶 糖基转移酶 |
| 文章编号： | 1009-0002(2004)03-0272-03 |
| 修稿时间： | 2004年3月8日 |
Advanced studies on the engineering of yeast asparagines-linked glycosylation |
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| ZHAN Jie,WU Jun Institute of Biotechnology,Academy of Military Medical Sciences,Beijing,China.Advanced studies on the engineering of yeast asparagines-linked glycosylation[J].Letters in Biotechnology,2004,15(3):272-274. |
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| Authors: | ZHAN Jie WU Jun Institute of Biotechnology Academy of Military Medical Sciences Beijing China |
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| Institution: | ZHAN Jie,WU Jun Institute of Biotechnology,Academy of Military Medical Sciences,Beijing100071,China |
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| Abstract: | s:Yeast expression system can be used to produce several recombinant glycoproteins with natural biological ac-tivity.But in the process of its N-glycosylation,hyper-mannose-type sugar chains are formed.By introducing and localiz-ing mannosidases and glycosyltransferases into the yeast,the pathway of hyperglycosylation are effectively blocked.In this paper,recent developments in the engineering of yeast N-glycosylation are reviewed,and some problems are also noted. |
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| Keywords: | yeast N-glycosylation hyper-mannose type mannosidase glycosyltransferase |
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