取食转基因抗虫棉上的棉蚜对粉舞蛛存活和发育的影响

本文以转双价基因棉花SGK321、棉蚜Aphis gossypii和粉舞蛛Alopecosa pulverulenta为对象，研究了捕食转基因植物上的植食性害虫对多食性捕食性天敌的影响。结果表明，粉舞蛛可以猎食棉蚜，但单独捕食棉蚜不足以长期维持若蛛的生存和发育； 与果蝇混合饲养，能显著提高若蛛存活率和体重。在猎物过量或数量不足的情况下，单独捕食转基因棉或常规棉上的棉蚜，若蛛的生存曲线和体重差异不显著。在猎物过量的条件下，用转基因棉上的棉蚜与果蝇混合饲养，若蛛的存活率显著高于用常规棉上的棉蚜与果蝇混合的处理； 但这两种处理下，若蛛的体重差异不显著。在猎物数量不足的情况下，用转基因棉或常规棉上的棉蚜与果蝇混合饲养，若蛛的存活率和体重差异都不显著。可见，转双价基因棉花SGK321上的棉蚜对粉舞蛛的存活和发育没有显著的不利影响。

Survival and development of the wolf spider Alopecosa pulverulenta feeding on cotton aphid Aphis gossypii propagated on transgenic cotton

Prey-mediated effects of genetically manipulated (GM) plants on polyphagous natural enemies were studied. Transgenic cotton cv. SGK321 (with inserted genes of Cry1Ac and CpTI) was used as the GM, the cotton aphid Aphis gossypii as the model pest, and the wolf spider Alopecosa pulverulenta (Clerck) as the generalist natural enemy. Aphids reared on GM vs. non-GM cotton were both poor quality food for A. pulverulenta spiderlings, in terms of growth and mortality. But the spiderlings showed higher survival and developed better when they were fed with aphids and fruit flies alternatively. When fed with aphids only, there were no differences in impact whether prey aphids were from GM or non-GM cotton, both in the limited feeding and unlimited feeding regimes. In the unlimited feeding experiment, the spiderlings showed higher survival when they were fed with aphids from GM cotton and fruit flies alternatively, compared with those fed with aphids from non-GM cotton and fruit flies alternatively, but body mass of the spiderlings did not differ between these two treatments. In the limited and mixed feeding experiment, there were no differences in impact whether prey aphids were from GM or non-GM cotton. The results indicate that transgenic cotton cv. SGK321 has no significant adverse effects on the survival and development of the wolf spider through non-target prey cotton aphid.