Fermentation of enzymatically saccharified sunflower stalks for ethanol production and its scale up |
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Authors: | Sharma Sanjeev K Kalra Krishan L Grewal Harmeet S |
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Institution: | Department of Microbiology, College of Basic Sciences and Humanities, Punjab Agricultural University, India. |
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Abstract: | Pretreated sunflower stalks saccharified with a Trichoderma reesei Rut-C 30 cellulase showed 57.8% saccharification. Enzyme hydrolysate concentrated to 40 g/l reducing sugars was fermented under optimum conditions of fermentation time (24 h), pH (5.0), temperature (30 degrees C) and inoculum size (3% v/v) and, showed a maximum ethanol yield of 0.444 g/g ethanol. Ethanol production scaled up in a 1 l and a 15 l fermenter under optimum conditions revealed maximum ethanol yields of 0.439 and 0.437 g/g respectively. |
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