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| Hela细胞端粒DNA断裂损伤 | |
| 引用本文： | 阳芳,杨洁萍,李清焕,邵兰,谭铮. Hela细胞端粒DNA断裂损伤[J]. 动物学报, 2003, 49(6): 873-877 |
| 作者姓名： | 阳芳 杨洁萍 李清焕 邵兰 谭铮 |
| 作者单位： | 1. 武汉大学生命科学学院,武汉,430072 2. 中国科学院动物研究所,北京,100080 3. 武汉大学生命科学学院,武汉,430072;中国科学院动物研究所,北京,100080 |
| 基金项目： | 中国科学院“百人计划”，“知识创新工程”项目资助 |
| 摘 要： | Telomeres are the repetitive G-rich DNA sequences at the end of chromosomes and shorten at each round of cell division.Besides the incomplete DNA synthesis,single and double DNA strand breaks,if not repaired, also contribute to the telomere shortening.To assess the frequency of strand breaks in proliferating Hela cells,telomere fragments were released by alkaline denaturing and electrophoresis from cells embedded in agarose,blotted onto membrane,and detected by probe specific to telomere sequence.The quantity of telomere fragments released was estimated to be less than 0.4% of the total telomere content,which corresponded to less than one break per cell.Since the mean length of the terminal restriction fragments of the cells was about 7 kbp,the fragments detected would lead to less than 19 bp in mean telomere shortening [Acta Zoologica Sinica 49(6):873-877,2003]. |
| 关 键 词： | 端粒 DNA断裂 Hela细胞 断裂损伤 染色体 |
Strand breaks in telomeres in Hela cells |
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| Abstract: | Telomeres are the repetitive G rich DNA sequences at the end of chromosomes and shorten at each round of cell division. Besides the incomplete DNA synthesis, single and double DNA strand breaks, if not repaired, also contribute to the telomere shortening. To assess the frequency of strand breaks in proliferating Hela cells, telomere fragments were released by alkaline denaturing and electrophoresis from cells embedded in agarose, blotted onto membrane, and detected by probe specific to telomere sequence. The quantity of telomere fragments released was estimated to be less than 0.4% of the total telomere content, which corresponded to less than one break per cell. Since the mean length of the terminal restriction fragments of the cells was about 7 kbp, the fragments detected would lead to less than 19 bp in mean telomere shortening. |
| Keywords: | Telomere DNA strand break Hela cells |
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