Ethyl methanesulfonate treatment of celery plants affects the expression pattern of CEL I endonuclease |
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| Authors: | Mitra Saffarian Tousi Ahmad Reza Bahrami Jafar Zolala Maryam M Matin |
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| Institution: | (1) Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, P.O. Box 9177948978, Mashhad, Iran;(2) Cell and Molecular Biology Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, P.O. Box 9177948974, Mashhad, Iran;(3) Department of Biotechnology, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran; |
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| Abstract: | CEL I enzyme from celery, as a member of S1 family of nucleases, is known for its high specific activity in recognition and
cleavage of base-substitution mismatches on heteroduplex DNA molecules. Despite valuable applications of the enzyme in mutation
screening studies, little is known about its function at cellular level. In the present study, we investigated the pattern
of CEL I expression in ethyl methanesulfonate (EMS) treated celery plants. An abnormal growth pattern along with wide and
clear lesions were observed on the treated plants. A considerable increase in the level of CEL I protein happened in vegetative
and generative parts of EMS-treated plants compared with controls. Despite such induction, the enzyme is not expected to be
involved in DNA repair during EMS treatment due to the absence of any known nuclear localization signal in the deduced sequence
of CEL I protein. Considering the fact that CEL I orthologs are induced during programmed cell death, the high expression
of CEL I upon EMS treatment could be due to the stress and necrotic cell death created by the treatment. |
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