Survival rate of preantral follicles derived from vitrified neonate mouse ovarian tissue by Cryotop and conventional methods |
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Authors: | Eimani H Mamzoji S S Soleimani M M Abnosi M H Valojerdi M Rezazadeh Yazdi P Eftekhari Shahverdi A Guorabi H |
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Affiliation: | Department of Embryology, Reproductive Medicine Center, Royan Institute, Tehran, Iran. Eimanih@yahoo.com |
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Abstract: | The aim of this study was to investigate the growth and survival rate of preantral follicles isolated from vitrified ovarian tissue by Cryotop and conventional methods. The ovaries of 14-day-old mice were separated and divided into four groups as following: Cryotop group, vitrified by Cryotop; CV (Conventional; CV) group, vitrified by conventional straw; toxicity test group and control group. After warming the vitrified ovaries, isolated preantral follicles from four groups were cultured for 4 days to compare survival rate and follicular growth between above-mentioned groups. Survival rate (97.3%) in toxicity test group alike the control group (98.7%) were significantly higher (P<0.05) than the Cryotop (92.7%) and CV (47.7%) groups. Increase in follicle diameters after 4 days in Cryotop and CV groups was significantly lower (P<0.05) than the control and toxicity test groups, but growth and survival rate of follicles in Cryotop group was significantly higher (P<0.05) than the CV group. These results demonstrated that ovarian tissue vitrification by Cryotop highly preserves the viability rate of preantral follicles. |
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