Immunological studies of ferredoxin-nitrite reductases and ferredoxin-glutamate synthases from photosynthetic organisms |
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Authors: | Cecilia Gotor Eloísa Pajuelo Luís C Romero Antonio J Márquez José M Vega |
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Institution: | (1) Departamento de Bioquímica Vegetal y Biología Molecular, Facultad de Química, Universidad de Sevilla, Apartado 553, España |
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Abstract: | Polyclonal antiserum specific for ferredoxin-nitrite reductase (EC 1.7.7.1) from the green alga Chlamydomonas reinhardii recognized the nitrite reductase from other green algae, but did not cross-react with the corresponding enzyme from different cyanobacteria or higher plant leaves. An analogous situation was also found for ferredoxin-glutamate synthase (EC 1.4.7.1), using its specific antiserum. Besides, the antibodies raised against C. reinhardii ferredoxin-glutamate synthase were able to inactivate the ferredoxin-dependent activity of nitrite reductase from green algae.These results suggest that there exist similar domains in ferredoxin-nitrite reductases and ferredoxin-glutamate synthases from green algae. In addition, both types of enzymes share common antigenic determinants, probably located at the ferredoxin-binding domain. In spite of their physicochemical resemblances, no apparent antigenic correlation exists between the corresponding enzymes from green algae and those from higher plant leaves or cyanobacteria.Abbreviations Fd
ferredoxin
- GOGAT
glutamate synthase
- MV+
reduced methyl viologen (radical cation)
- NiR
nitrite reductase
- PMSF
phenylmethylsulphonyl fluoride
- SDS
sodium dodecyl sulfate |
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Keywords: | Photosynthetic organisms Chlamydomonas reinhardii Ferredoxin-glutamate synthase Ferredoxin-nitrite reductase Common antigenic determinants Ferredoxin-binding domain |
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