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远志内生真菌分离鉴定及活性筛选
引用本文:崔晋龙,郭吉刚,范黎. 远志内生真菌分离鉴定及活性筛选[J]. 微生物学通报, 2007, 34(5): 0839-0842
作者姓名:崔晋龙  郭吉刚  范黎
作者单位:1. 首都师范大学生命科学学院,北京,100037
2. 山西生物应用职业技术学院,太原,030031
基金项目:致谢本实验得到本校吕萧、刘晶晶、刘少伟、王玉君等同学的大力支持和帮助,在此表示衷心感谢.
摘    要:从两个不同生长时期野生远志中分离内生真菌菌株88株,隶属于28个属,研究了各菌株对3种指示菌的拮抗作用。结果表明,远志不同生长时期不同部位的内生真菌数量、分布、种群存在差异,其优势属为Alternaria Nees。茎中内生真菌种类较多。88株内生真菌中有73株菌至少能拮抗1种指示菌,占总菌数的83.0%。4株抗性较强的菌株分别隶属于Trichothecium Link、Cephalosporium Corda、Alternaria Nees、Dactuliophora C.L.等。

关 键 词:远志  内生真菌  分离  鉴定  活性筛选
文章编号:0253-2654(2007)05-0839-04
修稿时间:2006-12-14

Isolation, Identification and Bioactivity Screening of Endophytic FungusAssociated with Polygala tenuifolia Willd
CUI Jin-Long,GUO Ji-Gang and FAN Li. Isolation, Identification and Bioactivity Screening of Endophytic FungusAssociated with Polygala tenuifolia Willd[J]. Microbiology China, 2007, 34(5): 0839-0842
Authors:CUI Jin-Long  GUO Ji-Gang  FAN Li
Affiliation:College of Life Sciences, Capital Normal University, Beijing 100037;Shanxi Biological Application Vocational Technical College, Taiyuan 030031;College of Life Sciences, Capital Normal University, Beijing 100037
Abstract:Eighty-eight strains of endophytic fungus,belonging to 28 genera, were isolated from wild Polygala tenuifolia Willd of two different growth stage,and the inhibitory activities screening to three species of microbe were conducted research. The results showed that the quantity, population and distribution of the endophytic fungi varied in different tissue and different growth stage of P. tenuifolia. The species of endophytic fungus distributed in stem were more than in root and leaf, and the dominant genus was Alternaria Nees. Seventy-three strains, accounting for 83.0% of all the isolates, presented antimicrobial activities to the tested indicators. High antimicrobial activities were found in four strains, which seperatedly belonged to the genera as follows: Trichothecium Link,Cephalosp-orium Corda,Alternaria Nees,Trichosporiella Kamyschko. et al.
Keywords:Polygala tenuifolia   Endophytic fungus   Isolation   Identification   Bioactivity screening
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