Reduction of protease inhibitor activity by expression of a mutant Bowman-Birk gene in soybean seed |
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| Authors: | Donald Livingstone Vadim Beilinson Marina Kalyaeva Monica A Schmidt Eliot M Herman Niels C Nielsen |
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| Institution: | (1) Agricultural Research Service, United States Department of Agriculture, St. Louis, USA;(2) Agricultural Research Service, United States Department of Agriculture, West Lafayette, USA;(3) Department of Agronomy, Purdue University, West Lafayette, IN 47907, USA;(4) Department of Biochemistry, Purdue University, West Lafayette, IN 47907, USA;(5) Donald Danforth Plant Science Center, 975 Warson Rd, St. Louis, MO 63132, USA;(6) Department of Crop Science, North Carolina State University, Raleigh, NC 27695, USA;(7) Athenix Corporation, 2202 Ellis Rd, Durham, NC 27703, USA;(8) J.R. Simplot Company, 999 Main St., Suite 1300, Boise, ID 83702, USA |
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| Abstract: | A mutant Bowman-Birk gene was created that encoded an inactive high-sulfur product. It was used to transform soybean line
Asgrow 3237. Transformants bearing the mutant gene were identified by GUS expression, PCR analysis, and Southern analysis.
The amount of steady state mRNA from the mutant gene in the transformed plants showed that the gene was highly expressed,
but the amount of message from the unmodified Bowman-Birk gene did not change detectably. Proteins synthesized at the direction
of the mutant Bowman-Birk gene accumulated in seeds of the transformed plants, and there was a marked decrease in the ability
of extracts prepared from these seeds to inhibit trypsin and chymotrypsin despite the presence of Kunitz trypsin inhibitor.
The more prevalent mRNA from the mutant gene was considered to out-compete message from the native genes to decrease the amount
of active Bowman-Birk inhibitor. |
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| Keywords: | Bowman-Birk Anti-nutritional Kunitz Transgenic Soybean Glycine max |
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