Translocation of colicin from the receptor to the inner cell membrane: function of the peptidoglycan layer |
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Authors: | Smarda J Mat?jková P Vavrícková A |
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Institution: | (1) Department of Biology, Faculty of Medicine, Masaryk University, 662 43 Brno, Czechia |
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Abstract: | Sensitivity of spheroplasts (prepared in two ways) of a colicin-sensitive strain, of colicinresistant and of colicin-tolerant
mutants and of strains immune to colicins E1 and E2 was estimated and compared. Generally, the removal of the peptidoglycan
layer brought about a slight nonspecific support for colicin translocation across the cell wall in sensitive,tolB tolerant and immune bacteria.tolB spheroplasts were colicin E1-sensitive, but E2-insensitive. Spheroplasts were always fragile and lysed spontaneously, especially
those produced by lysozyme. Bacteria carryingtolA, tolQ andtolR mutations kept their colicin insensitivity as spheroplasts, just as the resistant ones. Bacteria rendered colicinogenic and
hence colicin-immune turned to high colicin sensitivity in spheroplast form. The results indicate a change in plasma membrane
associated with the spheroplast formation. |
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