Fast preparative separation of 'native' core E coli 30S ribosomal proteins. |
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| Authors: | C Cachia P J Flamion J P Schreiber |
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| Affiliation: | Laboratoire de Biophysique, UFR des Sciences Pharmaceutiques et Biologiques, Dijon, France. |
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| Abstract: | We have developed an ion-exchange high performance liquid chromatographic method for preparative separation of 'core' proteins from E coli 30S ribosomal subunits, extracted with salt under non-denaturing conditions. This method yields individual proteins in pure and native form at high concentrations, (5 to 25 mg/ml) suitable for direct use in 1D-, 2D- or 3D-NMR studies. |
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