A model-based feeding strategy for fed-batch fermentation of recombinant Pichia pastoris |
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Authors: | MC dÕAnjou AJ Daugulis |
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Institution: | (1) Department of Chemical Engineering, QueenÕs University, Kingston, Ontario, Canada, K7L 3N6 |
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Abstract: | A two stage, exponential feeding strategy with mixed glycerol/methanol substrate was used in a fed-batch recombinant Pichia pastorisfermentation. The feeding strategy was developed using a simple model based on mass balances, Monod-type growth kinetics, and constant specific heterologous protein production rate. The model accurately predicted cell growth, and demonstrated the usefulness of a rational, model-based approach for improving the productivity of recombinant P. pastoris fermentation. |
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