Microcyst germination in the cellular slime mold, Polysphondylium pallidum: Effects of actinomycin D and cycloheximide on macromolecular synthesis and enzyme accumulation |
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Authors: | D. H. O'Day D. I. Gwynne D. H. Blakey |
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Abstract: | Germination of microcysts of Polysphondylium pallidum is characterized by an immediate rapid increase in incorporation of [3H]leucine into protein which is cycloheximide-sensitive but unaffected by actinomycin D. Significant RNA synthesis, as measured by [3H]uridine incorporation, does not begin until approx. 2 h after the onset of germination. The increase in [3H]uridine incorporation is prevented by actinomycin D. Germination and the increase in alkaline phosphatase and β-glucosidase enzyme activities are prevented by cycloheximide but unaffected by actinomycin D. The data strongly imply the presence of stable RNA in dormant microcysts and indicate a requirement for a discrete period of protein synthesis for germination of microcysts of P. pallidum. |
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