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Detection of vitellogenin incorporation into zebrafish oocytes by FITC fluorescence
Authors:Yohei Matsuda  Yoshie Ito  Hisashi Hashimoto  Hayato Yokoi  Tohru Suzuki
Affiliation:(1) Laboratory of Marine Life Science and Genetics, Graduate School of Agricultural Science, Tohoku University, Aoba-ku Sendai, 981-8555, Japan;(2) Bioscience and Biotechnology Center, Nagoya University, Nagoya 464-8601, Japan;(3) Industrial Technology Institute, Miyagi Prefectural Government, Sendai 981-3206, Japan
Abstract:

Background  

Large volumes of lymph can be collected from the eye-sacs of bubble-eye goldfish. We attempted to induce vitellogenin (Vtg) in the eye-sac lymph of bubble-eye goldfish and develop a method for visualizing Vtg incorporation by zebrafish oocytes using FITC-labeling.
Keywords:
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