He-Ne激光处理不同时期蚕豆幼苗对抗氧化系统的影响

采用功率为3.50 mW/mm2的He-Ne激光处理不同时期蚕豆,研究其四叶期叶片中丙二醛(MDA)含量,超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性及同工酶谱的变化。结果表明,与对照相比,He-Ne激光处理不同时期蚕豆,MDA含量均显著降低,且各处理间没有显著差异;SOD、POD、CAT酶活性有不同程度提高。SOD、POD同工酶的酶谱在浸种24小时和胚芽露头期经激光处理后改变,在一叶期处理后没有改变;CAT同工酶谱没有变化;在一叶期处理的三种同工酶谱都没有改变。

Changes of Anti-oxidative Enzymes in Vicia faba L. Seedlings Irradiated by He-Ne Laser in Different Stages

Variations in malondiadehycle(MDA) contents,enzymatic activities and isozymatic patterns of superoxide dismutases(SOD),peroxidases(POD) and catalases(CAT) of leaves at four-leaf stages were studied by treating Vicia faba L.with 3.50 mW/mm~2 He-Ne laser at different stages.The results indicated that He-Ne laser lead to a significant decrease in MDA contents,with no difference among treatment groups,while enzymatic activities of SOD,POD and CAT were enhanced by different degrees after Vicia faba L.being treated by He-Ne laser at different stages.In addition,isozymes changes of SOD and POD were observed in groups treated with laser after seeds being soaked for 24 h and when embryonic buds emerged,while no change was observed in the group treated at the one-leaf stage.There was no variation in CAT isozymes among different groups.Also,no changes in all three isozymes were observed in the group treated at the one-leaf stage.