Distinctive fibroblastic subpopulations in skin and oral mucosa demonstrated by differences in glycosaminoglycan content |
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Authors: | R E Bronson J G Argenta E P Siebert C N Bertolami |
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Institution: | (1) Department of Oral and Maxillofacial Surgery, Shriners Burns Institute, 02114 Boston, Massachusetts;(2) Massachusetts General Hospital, 02114 Boston, Massachusetts |
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Abstract: | Summary The glycosaminoglycan (GAG) content of rabbit skin, oral mucosa, and cultured 3H]-glucosamine-labeled dermal and submucosal fibroblasts was compared. Skin contained predominantly dermatan sulfate (DS)
and a small amount of hyaluronic acid (HA), whereas mucosa contained primarily keratan sulfate (KS) and smaller quantities
of HA and DS. Culture medium from dermal and submucosal fibroblasts contained GAGs co-electrophoresing with DS, HA, and chondroitin
sulfate (CS), although the relative proportions of these GAG differed. CS isolated from dermal and mucosal fibroblast culture
medium co-electrophoresed with chondroitin 4-sulfate (C4-S) on cellulose acetate, whereas dermal medium CS was resistant to
digestion by chondroitinase ABC, and mucosal medium CS was chondroitinase ABC-susceptible. The pericellular matrix of dermal
fibroblasts contained primarily DS and C4-S/C6-S, as confirmed by chondroitinase ABC digestion; the corresponding fraction
of mucosal fibroblasts contained HS and a GAG co-electrophoresing with a C6-S standard, yet resistant to digestion by chondroitinase
ABC. Thus the GAG content of dermal and mucosal fibroblasts differed both qualitatively in terms of the type of GAG secreted
into the culture medium and pericellular matrix, and quantitatively, in terms of the relative proportions of these GAGs in
both fractions. These differences support the concept of distinctive fibroblastic subpopulations in skin and mucosal tissue,
inasmuch as the cells were subjected to identical culturing conditions.
This work was supported by research grant 15878 (C.N.B.) from the Shriners Hospitals for Crippled Children and DE 07803 (C.N.B.)
from the National Institute of Dental Research, National Institutes of Health, Bethesda, MD. |
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Keywords: | fibroblasts glycosaminoglycans subpopulations mucosa |
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