Regeneration of foreign genes co-transformed plants of Medicago sativa L by Agrobacterium rhizogenes |
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Authors: | L Deyang CAO Xueyuan TANG Shunxue TIAN Xia |
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Institution: | Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China |
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Abstract: | Gene encoding sulphur amino acid-rich protein (HNP) and rol genes were transferred into Medicago sativa L (alfalfa) mediated by Agrobacterium tumafeciens. Regeneration of transgenic plants was induced successfully from hairy root tissue of cotyledon in alfalfa. Cotyledon tissues were an ideally transformed recipient. There was a negative correlation between age of hairy roots and embryogenesis frequency in alfalfa. Production of co-transformed plants with greater yield and super quality was important for development of new alfalfa varieties. |
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Keywords: | Medicago sativa HNP gene rol genes co-transformed plant |
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