Phylogeny of Chinese Allium (Liliaceae) using PCR-RFLP analysis |
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Authors: | HE Xingjin GE Song XU Jiemei HONG Deyuan |
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Affiliation: | 1. Biology Department, Sichuan University, Chengdu 610064, China 2. Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China |
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Abstract: | Eighteen representative species were selected from all the nine sections of Chinese Allium on the basis of the classification of morphology and cytotaxonomy. The trnK and rpL16 gene fragments of chloroplast DNA were amplified from 18 species by PCR method. The two cpDNA fragments were digested by 26 restriction enzymes, and 303 polymorphic restriction sites were found, of which 163 were informative. The restriction site data were analyzed with PAUP (version 3.1.1) and MEGA (version 1.01) as well as PHYLIP. As a result, the genus Allium could be classified into six subgenera. The recognition of Sect. Anguinum in the Flora of China is reasonable, Sect. Rhizirideum, Sect. Haplostemon and Sect. Cepa are not monophyletic. The infrageneric system of this genus was also discussed. |
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Keywords: | Allium molecular phylogeny PCR-RFLP trnK gene rpL16 gene |
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