Development and characterization of microsatellite markers for the endangered anchialine squat lobster Munidopsis polymorpha |
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| Authors: | P Cabezas P Bloor I Acevedo C Toledo M Calvo E Macpherson A Machordom |
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| Institution: | (1) Departamento de Biodiversidad y Biología Evolutiva, Museo Nacional de Ciencias Naturales (CSIC), c/José Gutiérrez Abascal, 2, 28006 Madrid, Spain;(2) Laboratorio de Ecología y Genética de Poblaciones, Centro de Ecología, Instituto Venezolano de Investigaciones Científicas, Apartado 20632, 1020 Caracas, Venezuela;(3) Departamento de Ecología Acuática, Centro de Estudios Avanzados de Blanes (CSIC), Carr. Acc. Cala Sant Francesc, 14, 17300 Blanes, Girona, Spain |
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| Abstract: | Species of the genus Munidopsis are typically distributed in bathyal and abyssal zones, but the anchialine species Munidopsis polymorpha is an exception. It inhabits a volcanic tube on Lanzarote Island (Canary Islands, NE Atlantic) and is currently listed as
endangered due to its highly restricted distribution and degree of endemism. Microsatellite loci were isolated from partial
genomic libraries that had been enriched for AC, ACAG, GATA, AAAC and AAG repeat sequences. Eight loci were polymorphic in
a sample of 24 individuals. The number of alleles per locus ranged from 2 to 4 with observed and expected heterozygosities
ranging from 0.083 to 0.875 and from 0.080 to 0.681, respectively. These markers will be used to evaluate levels of genetic
diversity and inbreeding, providing essential information for the development of a management and conservation strategy for
this species. |
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| Keywords: | Canary Islands Endangered species Microsatellites Munidopsis polymorpha |
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