Prokaryotic diversity of a Tunisian multipond solar saltern |
| |
Authors: | Houda Baati Sonda Guermazi Ridha Amdouni Neji Gharsallah Abdelghani Sghir Emna Ammar |
| |
Institution: | 1.Ecole Nationale d’Ingénieurs de Sfax, UR Etude et Gestion des Environnements C?tiers et Urbains,Sfax,Tunisia;2.Université d’Evry Val d’Essonne,Evry,France;3.CNRS-UMR 8030- CE,Evry,France;4.CEA- Genoscope,Le Pecq,France;5.Laboratoire d’Analyse,Compagnie Générale des Salines Tunisiennes (COTUSAL),Sfax,Tunisia;6.Laboratoire de Biotechnologie Microbienne,Faculté des Sciences de Sfax,Sfax,Tunisia |
| |
Abstract: | 16S rRNA gene clone libraries were separately constructed from three ponds with different salt concentrations, M2 (15%), TS38
(25%) and S5 (32%), located within a multipond solar saltern of Sfax. The 16S rRNA genes from 216 bacterial clones and 156
archaeal clones were sequenced and phylogenetically analyzed. 44 operational taxonomic units (OTUs) were generated for Bacteria and 67 for Archaea. Phylogenetic groups within the bacterial domain were restricted to Bacteroidetes and Proteobacteria, with the exception that one cyanobacterial OTU was found in the TS38 pond. 85.7, 26.6 and 25.0% of the bacterial OTUs from
M2, TS38 and S5 ponds, respectively, are novel. All archaeal 16S rRNA gene sequences were exclusively affiliated with Euryarchaeota. 75.0, 60.0 and 66.7% of the OTUs from, respectively, M2, TS38 and S5 ponds are novel. The result showed that the Tunisian
multipond solar saltern harbored novel prokaryotic diversity that has never been reported before for solar salterns. In addition,
diversity measurement indicated a decrease of bacterial diversity and an increase of archaeal diversity with rising salinity
gradient, which was in agreement with the previous observation for thalassohaline systems. Comparative analysis showed that
prokaryotic diversity of Tunisian saltern was higher than that of other salterns previously studied.
A. Sghir and E. Ammar have equally contributed to this work. |
| |
Keywords: | Solar saltern Molecular diversity Bacteria Archaea 16S rRNA genes |
本文献已被 PubMed SpringerLink 等数据库收录! |
|