Regulation of the synthesis of methanol oxidizing enzymes inKloeckera sp. 2201 andHansenula polymorpha,a comparison |
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| Authors: | Th Egli Th Haltmeier A Fiechter |
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| Institution: | (1) Swiss Federal Institute of Technology, ETH-Hönggerberg, CH-8093 Zürich, Switzerland;(2) Present address: Department of Microbiology, University of Sheffield, S10 2TN Sheffield, England |
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| Abstract: | A comparative study was made of the regulation of the synthesis of methanol dissimilating enzymes inkloeckera sp. 2201 andHansenula polymorpha using chemostat and batch growth conditions and methanol or glucose as carbon sources. During growth in methanol-limited chemostat cultures similar enzyme patterns for alcohol oxidase, catalase, formaldehyde dehydrogenase and formate dehydrogenase in the two yeasts were found. When growing in batch culture with glucoseH. polymorpha, but notKloeckera sp. 2201, was found to produce ethanol which might affect the synthesis of these enzymes. |
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| Keywords: | Hansenula polymorpha Kloeckera sp 2201 Methanol Glucose Methanol dissimilating enzymes Regulation |
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