Differential response of antioxidative enzymes in embryonic axes and cotyledons of germinating lupine seeds |
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Authors: | Ma?gorzata Garnczarska ?ukasz Wojtyla |
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Institution: | (1) Department of Plant Physiology, A. Mickiewicz University, Umultowska 89, 61-614 Poznan, Poland |
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Abstract: | Germination of lupine (Lupinus luteus L.) seeds was accompanied by an increase in concentration of free radicals with g
1
and g
2
values of 2.0056 ± 0.0003 and 2.0033 ± 0.0005, respectively. The highest intensity of free radical signal was observed in
embryo axes immediately after radicle protruded through the seed coat. Hydrogen peroxide accumulated in embryonic axes and
cotyledons during imbibition before the onset of germination in the seed population. The activities of superoxide dismutase
(SOD, EC 1.15.1.1) and catalase (CAT, EC 1.11.1.6) rose progressively in embryo axes. In cotyledons SOD activity did not change
significantly, while that of CAT increased during germination. The enhancement of Cu, Zn-SODs and Mn-SOD isoforms in embryonic
axes was observed. A new isoform of catalase was synthesized, suggesting that it plays a relevant role during germination.
SOD and CAT activities were detected in dry seeds. Free radical generation and response of antioxidative enzymes differed
between embryo axes and cotyledons during the germination timecourse. |
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Keywords: | Antioxidative enzymes Free radicals Germination Lupinus luteus L |
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