Abstract: | Before reaching the oxidase located inside the cell on the mitochondrial membrane, oxygen may be slowed down by diffusion within the cytoplasm. Diffusion or enzymic activity may predominate and this is related to the size and morphology of the organism, the intracellular diffusion coefficient, and the Km of the oxidizing terminal enzyme. This Km may be apparently diminished by inhibitors. Different experimental situations can arise in some plants (where oxygen diffusion in bulk tissue is not important in comparison to that in cell): either oxygen diffusion is limiting, or diffusion and an enzymic reaction compete, or diffusion does not slow down the respiratory rate. A mathematical model relates the oxygen concentration in the external medium, to the rate of oxygen uptake at the oxygen cytochrome-oxidase reaction level. |