Nystatin-, mycoheptin- and levorin-induced conductance in the membrane of frog skeletal muscle fibres |
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Authors: | N E Shvinka G Caffier |
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Institution: | (1) the Russian Academy of Sciences, Institute of Cytology, Tikhoretsky pr. 4, 194064 St. Petersburg, Russia;(2) Department of Physiology of Work, Central Institute of Occupational Medicine, Berlin, Germany |
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Abstract: | The effects of the polyene antibiotics nystatin (2 × 10–5–10–4 mol/l), mycoheptin (1.3 × 10–6–10–5 mol/l) and levorin (10–8–5 × 10–5 mol/l)on isolated frog skeletal muscle fibres and whole sartorius muscles of the frog have been investigated. Cation conductance was measured under current clamp conditions using a double sucrosegap technique. Cation effluxes were studied by means of flame emission photometry. All three antibiotics increased the cation conductance and efflux rates; however, differences between the polyenes were found in the steady state values of induced cation transport at a given concentration. The values of both induced conductance gA
and efflux rate constants KA formed the following sequence: levorin > mycoheptin > nystatin, demonstrating a correlation with the order of antifungal activities. The dose-response curves of lg polyene-induced cation transport against lg of antibiotic concentration in our experiments had slope values which were much lower than those in bilayers: 1.7 and 1.3 for nystatin and mycoheptin, respectively, whereas the aromatic heptaene levorin had an even smaller concentration dependence. The decline in the equilibrium conductance caused by nystatin- and mycoheptin removal was very fast (during the first minute = 0.74 and 2.39 min, respectively). In contrast, levorin-induced conductance was irreversible. It is proposed that the processes which limit the rate of channel formation are different in biological and model membranes.
Correspondence to: N. E. Shvinka |
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Keywords: | Polyene antibiotics Cation conductance Cation effluxes Muscle fibre membrane |
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