In-vivo formation of xyloglucan nonasaccharide: A possible biologically active cell-wall fragment |
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Authors: | S C Fry |
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Institution: | (1) Department of Botany, University of Edinburgh, The King's Buildings, Mayfield Road, EH9 3JH Edinburgh, UK |
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Abstract: | The in-vivo formation of a specific nonasaccharide of xyloglucan was investigated. This nonasaccharide has been reported to have biological activity, inhibiting auxin-induced growth in pea stem segments. Cell-suspension cultures of spinach were grown in the presence of 3H]arabinose and 3H]fucose, and the culture-filtrates were examined for oligosaccharides by gelpermeation chromatography and by paper chromatography. Sixteen 3H]pentose-containing oligosaccharides were found, including twelve that contained the sequence 3H]xylosyl- (1 6)-glucose, which is diagnostic of xyloglucan. In addition, 3H]fucose-containing oligosaccharides of at least three sizes were found. Radiochemical evidence is presented that one of these oligosaccharides was labelled with both 3H]fucose and with 3H]pentose, and was identical with the major xyloglucan-derived nonasaccharide associated with anti-auxin activity. It was largely present in the form of acylated (possibly acetylated) derivatives. It accumulated extracellularly to a steady-state concentration of about 4.3·10-7M. This is the first report of the production of a biologically-active oligosaccharide by living plant cells.Abbreviations BAB
butanone/acetic acid/H3BO3-saturated water (9:1:1)
- BAW
butan-1-ol/acetic acid/water (12:3:5)
- BPW
butan-1-ol/pyridine/water/(4:3:4)
- DP
degree of polymerisation
- FAW
ethyl acetate/acetic acid/water (10:5:6)
- EPW
ethyl acetate/pyridine/water (8:2:1)
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k
av
elution volume relative to Blue Dextran (k
av.=0.0) and glucose (k
av.=1.0)
- XG7
XG9 minus the fucose and galactose residues
- XG9
the particular xyloglucan nonasaccharide illustrated in Fig. 1
- W
water-saturated phenol |
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Keywords: | Anti-auxin Cell wall (active fragment) Nonasaccharide Oligosaccharin gif" alt="ldquo" align="MIDDLE" BORDER="0">Oligosaccharin " target="_blank">gif" alt="rdquo" align="MIDDLE" BORDER="0"> Spinacia (xyloglucan) Xyloglucan |
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