Methods of preparing well-orientated sols of f-actin containing filaments suitable for X-ray diffraction |
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Authors: | D Popp V V Lednev W Jahn |
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Affiliation: | Max Planck Institute for Medical Research, Heidelberg, Federal Republic of Germany. |
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Abstract: | In this paper we describe methods of preparing orientated f-actin and reconstituting thin filaments that are suitable for X-ray diffraction that allow us to analyse the structure of f-actin to at least 15 A resolution (1 A = 0.1 nm). We described problems that occur during the process of orientation and ways of solving them. |
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