浅水应激后黄斑蓝子鱼生理指标变化及牛磺酸的抗应激作用

研究报道黄斑蓝子鱼受到短暂（4min）浅水应激后鱼体相关应激指标的变化及应激后20、40、60、80min时的恢复情况，以及牛磺酸的抗应激作用。结果表明，浅水应激后，该鱼血清肾上腺素浓度显著升高（P0.05），从应激前的（2.000.22）ng/mL达到应激后的（15.121.04）ng/mL，之后逐渐恢复到应激前水平；皮质醇和胆固醇浓度没有显著性变化（P0.05）；葡萄糖浓度在应激后20min达到最大值（7.100.38）mmol/L，是应激前（2.150.02）mmol/L的约3.5倍（P0.05）。应激后60min时，脑中HSP70 mRNA的表达量达到最大值，为应激前的11.54倍（P0.05）。0.02牛磺酸浸泡能够显著降低蓝子鱼应激后的血清肾上腺素和葡萄糖水平以及脑HSP70 mRNA表达量（P0.05）。结果说明，交感神经－嗜铬组织系统可能是黄斑蓝子鱼的急性应激途径之一，鱼体通过释放肾上腺素来提高血糖浓度以满足应激时的能量需求，同时通过提高细胞HSP70的表达水平来增强鱼体的保护作用；牛磺酸具有一定的抗应激作用。  

PHYSIOLOGICAL CHANGES IN SIGANUS CANALICULATUS AFTER SHALLOW WATER STRESS AND THE ANTI-STRESS EFFECTS OF TAURINE

In this study several physiological indicators to stress of rabbitfish were investigated after the subject were shortly (4min) exposed to the shallow water stress. The recovery process were studied at 20, 40, 60, 80min post stress. In addition, the anti-stress effects of taurine were evaluated. The results showed that the serum adrenaline level was significantly increased from (2.000.22) ng/mL to (15.121.04) ng/mL after the exposure to stress (P0.05), and then gradually returned back to basal levels. No significant changes were observed in serum cortisol and cholesterol concentrations (P0.05). The serum glucose level reached the maximum level (7.100.38) mmol/L at 20min post the stress, which was 3.5 times higher than the basal level (P0.05). The expression of HSP70 mRNA in brain reached the maximum level at 60min post the stress, which was 11.54 times higher than the basal level (P0.05). Besides, the incubation with 20 ppm taurine significantly reduced the serum adrenaline, glucose and brain HSP70 mRNA levels after stress (P0.05). These results suggested that sympathetico-chromaffin system might be, if not all, at least one of the apparatus that account for rabbitfishs response to the acute stress. During this process adrenaline is released to elevate the blood glucose level which results in the boost in the energy. The increase in brain HSP70 expression also enhances the protective effect of organism. Noticeably taurine has the anti-stress effects in rabbitfish to certain extent.