A histochemical study of anionic sites in the intermediate layer of rat femoral cartilage using polyethyleneimine at different pH levels |
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Authors: | Hideho Ueda Hiroshi Toriumi Chong-Guang Leng Shinichi Ohno |
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Institution: | (1) Department of Anatomy, Yamanashi Medical University Tamaho, Yamanashi, Japan |
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Abstract: | Anionic sites in the intermediate layer of young rat hyaline cartilages were examined using a cationic dye, polyethyleneimine
(PEI), at different pH levels. Femoral heads were resected and fixed in 2.5% glutaraldehyde and treated with 0.5% PEI at pH
7.4, pH 2.5 or pH 1.0. Some cartilage samples were first digested with chondroitinase ABC or hyaluronidase. The PEI deposits
at pH 7.4 appeared to be irregular shapes. Their sizes seemed to be larger than those at pH 2.5 or pH 1.0. The PEI deposits
were also found on the surface of collagen fibrils at both pH 7.4 and pH 2.5 even after the chondroitinase ABC digestion,
but were not found at pH 1.0. Moreover, they disappeared after hyaluronidase digestion. Accordingly, it is suggested that
PEI-positive structures varied depending on pH levels. In addition, hyaluronan may be localized near collagen fibrils, but
most sulphated proteoglycans may not.
This revised version was published online in November 2006 with corrections to the Cover Date. |
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