A dam mutant of Yersinia pestis is attenuated and induces protection against plague |
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| Authors: | Robinson Victoria L Oyston Petra C F Titball Richard W |
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| Affiliation: | Defence Science and Technology Laboratory, Biomedical Sciences, Salisbury, Wilts, UK. vlrobinson@dstl.gov.uk |
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| Abstract: | We have constructed a dam mutant of Yersinia pestis GB. In BALB/c mice inoculated subcutaneously, the median lethal dose of the mutant was at least 2000-fold higher than the wild type. Mice inoculated with sub-lethal doses of the mutant were protected against a subsequent challenge with virulent Y. pestis. The effect of dam inactivation on gene expression was examined using a DNA microarray, which revealed increased expression of a number of genes associated with the SOS response. These results confirm the key role of Dam in the regulation of virulence, and its potential role as a target for the generation of attenuated strains of pathogenic bacteria. |
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| Keywords: | DNA adenine methylase Plague Vaccine |
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