Quantitative detection of Listeria monocytogenes in biofilms by real-time PCR |
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| Authors: | Guilbaud Morgan de Coppet Pierre Bourion Fabrice Rachman Cinta Prévost Hervé Dousset Xavier |
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| Affiliation: | Laboratoire de Microbiologie Alimentaire et Industrielle, ENITIAA, Nantes, France. |
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| Abstract: | A quantitative method based on a real-time PCR assay to enumerate Listeria monocytogenes in biofilms was developed. The specificity for L. monocytogenes of primers targeting the listeriolysin gene was demonstrated using a SYBR Green I real-time PCR assay. The number of L. monocytogenes detected growing in biofilms was 6 x 10(2) CFU/cm2. |
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