Continuous requirement of cAMP for pre-spore differentiation in Dictyostelium discoideum |
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Authors: | Koji Okamoto |
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Affiliation: | Department of Botany, Faculty of Science, Kyoto University Sakyo-ku, Kyoto 606, Japan |
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Abstract: | Abstract Using a shaking culture system, we have previously shown that both cell contact and cAMP are required for pre-spore differentiation in Dictyostelium discoideum [2]. In the present study, cAMP was removed from the medium by the use of a hydrolysing enzyme after cells had formed agglomerates. This treatment left the agglomerates unchanged, but caused a rapid decrease in the activity of UDP galactose transferase, a pre-spore-specific enzyme. This result indicates that cAMP is required even after agglomerate formation to maintain pre-spore differentiation. |
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Keywords: | Dictyostelium discoideum differentiation cell contact cyclic AMP |
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