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A macrophage-tropic HIV-1 that expresses green fluorescent protein and infects alveolar and blood monocyte-derived macrophages |
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| Authors: | Elizabeth A. Rich Jan M. Orenstein Dr. Kuan-Teh Jeang |
| Affiliation: | (1) Department of Medicine, Case Western Reserve University, Cleveland, Ohio;(2) Department of Pathology, George Washington University, Washington, D.C.;(3) Molecular Virology Section, Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Md., USA |
| Abstract: | We describe the construction of a macrophage-tropic HIV-1 molecular clone, pNLAD8-EGFP, which expresses enhanced green fluorescent protein. We show that NLAD8-EGFP can infect monocyte-derived macrophages as well as alveolar macrophages. NLAD8-EGFP-infected macrophages can be easily and sensitively detected based on the visualization of intracellular green fluorescent protein. |
| Keywords: | HIV-1 Alveolar macrophages Blood monocytes Enhanced green fluorescent protein AIDS Cigarette smoking |
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